M. A. Cassidy, N. Crockett, F. J. Leeper and A. R. Battersby
"Synthetic Studies on the Proposed Spiro Intermediate for Biosynthesis of the Natural Porphyrins: The Stereochemical Probe"
J. Chem. Soc., Chem. Commun.,1991, 384-386.
The intermediacy of the spiro-pyrrolenine for biosynthesis of uro'gen III is supported by synthesis of both enantiomers of the spiro-lactam followed by demonstration that cosynthetase, the enzyme which forms uro'gen III, is inhibited over twenty times more strongly by one enantiomer than by the other.
Department of Chemistry
University of Cambridge